DOPAMINE D1A RECPT; RB X-50UG

Application

Research CategoryNeuroscience

Research Sub CategoryNeurotransmitters & Receptors

Western blot: 1-10 µg/mL (Chemiluminescence technique). An antibody made to this peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes. The 75 kDa band was not visible in striatum (Huang et al. 1992; O′Connel et al. 1995). Approximately 100-400 µg of protein/lane should be loaded to visualize the 75 kDa band (Huang et al. 1992; O′Connel et al. 1995) that is present in relatively small amounts.

Immunohistochemistry: 2-10 µg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 µg/mL (1 µg/mL D1A peptide (Cat. Number AG259)/well)

Optimal working dilutions must be determined by end user.

Anti-Dopamine D1A Receptor Antibody, C-terminus, cytoplasmic is an antibody against Dopamine D1A Receptor for use in ELISA, IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

A 13 amino acid peptide sequence (amino acids 403-415) from rat D1A (Monsma et al. 1990; Zhou et al. 1990; Zhou et al. 1992; O′Dowd et al. 1990). This peptide is predicted to be cytoplasmic.

Epitope: C-terminus, cytoplasmic

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified immunoglobulin. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% BSA.

Specificity

Dopamine Receptor D1A. An antibody made to the D1R peptide has been shown to detect 2 proteins of 50 and 75 kDa in solubilized hippocampal membranes by Western blot. The 75 kDa band was not visible in striatum (Huang et al. 1992). Approximately 100-400 µg of protein/lane should be loaded to visualize the 75 kDa band. By immunohistochemistry D1R has been localized in various regions of the rat brain (Huang et al. 1992).

May recognize porcine or bovine (100 % similarity) or human (78% similarity).

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.